Generator

Part:BBa_K2014004:Design

Designed by: Daria Niewiadomska   Group: iGEM16_UAM_Poznan   (2016-10-12)


pxylS-M5'UTR->sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 162


Design Notes

We decided to work on xylose induced promoter cause this sugar is relatively cheap.


Source

Xylose induced promoter is a fragment of E.coli K-12 genome. Synthetic 5'UTR is a synthetic unstructured sequence with well positioned RBS.

References